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Tools of the Laboratory: The Methods for Studying Microorganisms
Multiple Choice Questions
1. The Five I’s of studying microorganisms include all of the following except
A. inoculation.
B. incubation.
C. infection.
D. isolation.
E. identification.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
2. The term that refers to the purposeful addition of microorganisms into a laboratory nutrient
medium is
A. isolation.
B. inoculation.
C. immunization.
D. infection.
E. contamination.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-2
3. A pure culture contains
A. only one species of microorganism.
B. only bacteria.
C. a variety of microbes from one source.
D. a variety of species from the same genus.
E. None of the choices is correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
4. The correct microbiological term for the tiny sample of specimen that is put into a nutrient
medium in order to produce a culture is the
A. colony.
B. inoculum.
C. streak.
D. loop.
E. None of the choices is correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
5. Which of the following is essential for development of discrete, isolated colonies?
A. broth medium
B. differential medium
C. selective medium
D. solid medium
E. assay medium
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-3
6. Which method often results in colonies developing down throughout the agar along with
some colonies on the surface?
A. streak plate
B. spread plate
C. pour plate
D. All of the choices are correct.
E. None of the choices is correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
7. What type of isolation technique is most effective for the majority of applications?
A. pour plate
B. streak plate
C. spread plate
D. loop dilution
E. culture plate
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
8. Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled
and cooled?
A. a pure culture
B. a mixed culture
C. a solid medium
D. a liquid medium
E. a contaminated medium
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-4
9. Agar is an important component of media because
A. bacteria require agar to grow.
B. agar inhibits mold growth.
C. agar provides a solid surface for bacterial growth.
D. agar prevents contamination.
E. All of the choices are correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
10. The three physical forms of laboratory media are
A. solid, liquid, and gas.
B. solid, semisolid, and liquid.
C. streak plate, pour plate, and broth.
D. aerobic, anaerobic, and micro aerobic.
E. None of the choices is correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
11. Which of the following is not an inoculating tool?
A. petri dish
B. loop
C. needle
D. pipette
E. swab
ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-5
12. Agar is a complex polysaccharide that comes from a/an
A. green plant.
B. fungus.
C. mold.
D. algae.
E. euglena.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
13. Which of the following is not a benefit of agar as a solid medium?
A. flexibility
B. holds moisture
C. can be inoculated and poured at a temperature that is not harmful
D. solid at room temperature
E. is digested by most microbes
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
14. A nutrient medium that has all of its chemical components identified and their precise
concentrations known and reproducible, would be termed
A. complex.
B. reducing.
C. enriched.
D. chemically-defined.
E. None of the choices is correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-6
15. A nutrient medium that contains at least one ingredient that is NOT chemically definable
would be termed
A. complex.
B. reducing.
C. enriched.
D. synthetic.
E. None of the choices is correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
16. All of the following are examples of different types of microbiological media except
A. broth.
B. enriched.
C. agar.
D. petri dish.
E. selective.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-7
17. A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a
culture medium. Following incubation, only the E. coli grows in the culture. What is the most
likely explanation?
A. The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.
ASM Objective: 07.01b Ability to apply the process of science: Analyze and interpret results from a variety of microbiological methods, and
apply these methods to analogous situations.
ASM Topic: Module 07 Scientific Thinking
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
18. A common medium used for growing fastidious bacteria is
A. blood agar.
B. trypticase soy agar.
C. mannitol salt agar.
D. MacConkey medium.
E. a reducing medium.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-8
19. A reducing medium contains
A. sugars that can be fermented.
B. extra oxygen.
C. hemoglobin, vitamins, or other growth factors.
D. substances that remove oxygen.
E. inhibiting agents.
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
20. Which type of medium is able to distinguish different species or types of microorganisms
based on an observable change in the colonies or in the medium?
A. differential
B. selective
C. enumeration
D. enriched
E. reducing
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
21. A microbiologist decides to use a nutrient medium that contains thioglycollic acid. What
type of microbe is she attempting to culture?
A. fastidious
B. gram-positive
C. anaerobic
D. gram-negative
E. aerobic
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-9
22. Differential media results in which of the following growth characteristics?
A. different color colonies
B. different media color post incubation
C. precipitates
D. gas bubbles
E. All of the choices are correct.
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
23. A reducing media is used to culture
A. fastidious organisms.
B. aerobic organisms.
C. anaerobic organisms.
D. any pathogenic organisms.
E. None of the choices is correct.
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
24. For which bacterial genus does mannitol salt agar differentiate between species?
A. Salmonella
B. Streptococcus
C. Neisseria
D. Staphylococcus
E. Escherichia
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-10
25. A microbiologist must culture a patient’s feces for intestinal pathogens. Which of the
following would likely be present in selective media for analyzing this fecal specimen?
A. NaCl
B. sheep red blood cells
C. bile salts
D. thioglycollic acid
E. peptone
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
26. Bacteria that require special growth factors and complex nutrients are termed
A. aerobic.
B. anaerobic.
C. fastidious.
D. microaerophilic.
E. autotrophic.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-11
27. A microbiologist inoculates Staphylococcus aureus into a culture medium. Following
incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be
growing in this culture. What is the most likely explanation?
A. The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.
ASM Objective: 07.01b Ability to apply the process of science: Analyze and interpret results from a variety of microbiological methods, and
apply these methods to analogous situations.
ASM Topic: Module 07 Scientific Thinking
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
28. Newly inoculated cultures must be _____ at a specific temperature to encourage growth.
A. streaked
B. poured
C. incubated
D. contaminated
E. All of the choices are correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-12
29. The _____ of the microscope holds and allows selection of the objective lenses.
A. stage
B. condenser
C. objective
D. ocular
E. nosepiece
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
30. Which of the following will converge light rays to a single focal point on the specimen?
A. ocular lens
B. objective lens
C. iris diaphragm
D. condenser
E. nosepiece
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
31. Which of the following magnifies the specimen to produce the real image of the
specimen?
A. condenser
B. objective lens
C. ocular lens
D. body
E. nosepiece
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-13
32. Which of the following magnifies the specimen to produce the virtual image of the
specimen?
A. objective lens
B. ocular lens
C. condenser
D. body
E. iris diaphragm
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
33. Which of the following controls the amount of light entering the specimen?
A. objective lens
B. ocular lens
C. condenser
D. body
E. iris diaphragm
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
34. If a microbiologist is studying a specimen at a total magnification of 950x, what is the
magnifying power of the objective lens if the ocular lens is 10x?
A. 100x
B. 950x
C. 85x
D. 850x
E. 95x
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 02. Understand
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-14
35. Magnification is achieved in a compound microscope through the initial magnification of
the specimen by the _____ lens. This image is then projected to the _____ lens that will
further magnify the specimen to form a virtual image received by the eye.
A. ocular, objective
B. scanning, objective
C. objective, ocular
D. ocular, oil
E. None of the choices is correct.
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
36. Which of the following characteristics refers to the microscope’s ability to show two
separate entities as separate and distinct?
A. resolving power
B. magnification
C. refraction
D. All of the choices are correct.
E. None of the choices is correct.
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-15
37. All of the following are diameters of cells that would be resolved in a microscope with a
limit of resolution of 0.2µm except
A. 0.2 µm.
B. 0.2 mm.
C. 0.1 µm.
D. 0.3 µm.
E. 2.0 µm.
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 02. Understand
Learning Objective: 02.04 Convert among different lengths within the metric system.
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
38. The type of microscope in which you would see brightly illuminated specimens against a
black background is
A. bright-field.
B. dark-field.
C. phase-contrast.
D. fluorescence.
E. electron.
ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-16
39. Which type of microscope shows cells against a bright background and also shows
intracellular structures of unstained cells based on their varying densities?
A. bright-field
B. dark-field
C. phase-contrast
D. differential interference
E. electron
ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
40. Which type of microscope is the most widely used and shows cells against a bright
background?
A. bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.05 List and describe the three elements of good microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
41. All of the following pertain to the fluorescence microscope except
A. uses electrons to produce a specimen image.
B. is a type of compound microscope.
C. requires the use of dyes like acridine and fluoresce.
D. is commonly used to diagnose certain infections.
E. requires an ultraviolet radiation source.
ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.06 Differentiate between the principles of light and electron microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-17
42. A confocal scanning optical microscope
A. uses ultraviolet light to form a specimen image.
B. shows three-dimensional cell images from the cell surface to the middle of the cell.
C. produces specimen images on electron micrographs.
D. uses dyes that emit visible light when bombarded by ultraviolet rays.
E. requires specimens to be stained.
ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.06 Differentiate between the principles of light and electron microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
43. Which type of microscope does not use light in forming the specimen image?
A. bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.06 Differentiate between the principles of light and electron microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
44. Which type of microscope achieves the greatest resolution and highest magnification?
A. bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.06 Differentiate between the principles of light and electron microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-18
45. Which type of microscope bombards a whole, metal-coated specimen with electrons
moving back and forth over it?
A. fluorescence
B. differential interference contrast
C. scanning electron
D. transmission electron
E. phase-contrast
ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.06 Differentiate between the principles of light and electron microscopy.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
46. The specimen preparation that is best for viewing cell motility is
A. hanging drop.
B. fixed stained smear.
C. Gram stain.
D. negative stain.
E. flagellar stain.
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.07 Name the two main categories of stains.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
47. The purpose of staining cells on a microscope slide is to
A. kill them.
B. secure them to the slide.
C. enlarge the cells.
D. add contrast in order to see them better.
E. see motility.
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.07 Name the two main categories of stains.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-19
48. What do the Gram stain, acid-fast stain, and endospore stain have in common?
A. used on a wet mount of the specimen
B. use heat to force the dye into cell structures
C. outcome based on cell wall differences
D. use a negative stain technique
E. are simple stains
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.08 Give examples of a simple, differential, and special stain.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
49. Basic dyes are
A. attracted to the negatively charged acidic substances of bacterial cells.
B. anionic.
C. used in negative staining.
D. repelled by cells.
E. dyes such as India ink and nigrosin.
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.07 Name the two main categories of stains.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
50. A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler’s
methylene blue. All the cells appear blue under the oil lens. This is an example of
A. negative staining.
B. using an acidic dye.
C. simple staining.
D. using the acid-fast stain.
E. capsule staining.
ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 02. Understand
Learning Objective: 02.08 Give examples of a simple, differential, and special stain.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Chapter 02 – Tools of the Laboratory: The Methods for Studying Microorganisms
2-20
Matching Questions
51. Matching.
1.) 1 Km A.) 1 cm
2.) 10 mm B.) 100 mm
3.) 10 cm C.) 1000 mm
4.) 1 m D.) 1000 m
Ans:1. D 2. A 3. B 4. C
Bloom’s Level: 01. Remember
Learning Objective: 02.04 Convert among different lengths within the metric system.
Section: 02.02
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
Multiple Choice Questions
52. Which type of media would be the best choice when shipping a sample of bacteria to a
laboratory to be tested from a satellite office site?
A. transport
B. EMB
C. blood
D. thioglycollate
E. general purpose
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms